Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

Abstract

A multiwalled carbon nanotube – chitosan (MWCNT-Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5-hydroxytryptamine (5-HT). Electroanalytical response of the fabricated electrode towards the detection of 5-HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT-Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5 108 –1.6 105 M and the low-detection-limit is 5 108 M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5-HT in-vitro directly from artificial urine samples and from diluted serum samples.