Influence of dilute acid and alkali pretreatment on reducing sugar production from corncobs by crude enzymatic method: A comparative study

Abstract

In the present study, two commonly used catalysts in chemical pretreatment, sulfuric acid and sodium hydroxide, were tested to evaluate the effect of solid-to-liquid ratio on pretreatment and enzymatic hydrolysis. Solid to liquid ratio (S/L) was influential on sugars released with an increase in the S/L ratio between 0.03 and 0.2. Enzymatic digestibility of 0.25 M H2SO4 pretreated corncobs were released more sugars (415.12 mg/mL); whereas, corncobs pretreated with NaOH released 350.12 mg/mL of reducing sugars at S/L 0.05. Further, in comparison with NaOH pretreated corncobs, acid treated material substantially increased the accessibility and digestibility of cellulose during crude enzymatic hydrolysis (28.96 FPU) and released 398.95 mg/mL reducing sugars.