SIMPLE AND FACILE SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF AMISULPRIDE IN PHARMACEUTICAL FORMULATIONS

Abstract

Three simple, sensitive and novel spectrophotometric methods have been established for determination of amisulpride (ASP) in pharmaceutical dosage forms. The proposed methods are based on the diazotization of ASP with sodium nitrite and hydrochloric acid, followed by coupling with ethyl acetoacetate, 1-benzoyl acetone and 8- hydroxyquinoline in alkaline medium for methods A, B and C respectively. The formed azo dyes are measured spectro-photometrically at 438, 446 and 545 nm for methods A, B and C respectively. Different variables affecting the reactions were optimized. Beer’s law is obeyed over the concentration ranges of 1.0-24.0, 1.0-24.0 and 1.0-20.0 μg/mL for methods A, B, and C, respectively. The results of the proposed methods are validated statistically. The proposed methods were applied successfully to the determination of ASP in pharmaceutical dosage forms.