Studies on Phenol degradation using Pseudomonas putida in Fluidized bed Bioreactor: A Waste water treatment

Abstract

The present investigation deals with the biological degradation of Phenolic bulk drug waste with Pseudomonas putida, a Phenol degrading microorganism in a draft tube fluidized bed bioreactor and estimation of phenol transfer rates from bulk liquid to bioparticle. The concentration of highly harmful Phenol is found very much in the wastewaters of most of the Chemical and Pharmaceutical industries. The Pseudomonas putida was immobilized on plastic beads in a draft tube Fluidized bed bioreactor. Experiments have been conducted at different feed flow rates, feed concentrations and air flow rates. The temperature and PH were maintained at 7.0 at 300C respectively in all the runs. The concentration of phenol in the outlet of rector was analyzed after every one hour until steady state is reached. The exponential growth phases of the microorganism and the Biokinetic parameters have been determined. The transfer rates of Phenol through liquid phase to bioparticle for different feed flow rates, feed concentrations and air flow rates have also determined. Key words: Phenolic Bulk drug effluent, pseudomonas putida, Mass transfer studies, mathematical modeling and Fluidized bed bioreacto